Sunday, February 27, 2011

The Effect of Euphorbia Hirta L. to Animal Intestinal Parasites

Mercader, Arlene L.
Mescallado, Kim Leianne M.
Montebon, Mariel S.
Sagana, Patricia Anne B.
Boquiren, Richard M.
Research Adviser:
Mrs. Ma. Regaele A. Olarte
           Euphorbia Hirta L. or so-called Tawa tawa is a plant abundantly growing in almost all parts of the Philippines and is usually taken for granted due to its undiscovered scientific and medicinal importance. Recently, it was said that it is used in some parts of our country to increase the platelet count of dengue patients. Further studies were conducted to prove its effectiveness.
This research used Euphorbia Hirta L. but it was aimed at the decoction and utilization of the said plant as an animal purgative. Fifty milligrams of Euphorbia Hirta L. samples were gathered and the removal of unnecessary parts was done. Three different amounts of stem pieces which were about 1 cm. long were subjected to heat together with the 20 ml. of distilled water. The decocted products were cooled. Fifteen mice were used to test the decocted products and a commercialized purgative and were kept under observation for 12 hours in fifteen different shoe boxes.
The results were gathered by studying the feces of each mouse obtained from the shoe boxes after the said time. Unpaired t test was used for the statistical analysis. The results showed that there was a direct relationship between the amount of Euphorbia Hirta L. and the number of intestinal parasites purged and the effectiveness of each concentration depends on the amount of Euphorbia Hirta L.
Based on the findings obtained, the researchers concluded that Euphorbia Hirta L. is an effective animal purgative. The researchers recommended further experiments to strengthen this study and to discover the other medicinal uses and contributions of this plant.

The Effect of the Varying Concentration of Carica Papaya Seeds Extract to the Albino-White Mice Sperm Cell

Researcher: Darren Lambert Bautista
Research Adviser: Mrs. Ma. Regaele A. Olarte

 Albino white mice sperm cells

  Papaya seeds

Methods and Procedure
            After the essential materials were gathered the researcher performed the procedural ways for the extraction of the papaya seeds. The collected papaya seeds was air-dried for 48 hours and was ground until it was powdered using a mortar and pestle. 1000 grams  were weighed using a digital balance and placed in an Erlenmeyer flask; it was treated with sufficient solvent with the ratio of 1:1 to completely immerse the seeds in the solution. A stopper was used to cover the flask and the solution was left for 24 hours (enough time for the powdered seeds to mix with the solvent.)
The filtrate was then filtered using a filter paper and a beaker and the residue of the solution was properly disposed.
Then the researcher measured the extract in milliliters and administered to the cell. The researcher prepared four set ups for the experiment, 3 were the experimental set ups and 1 was the control group. The first set up was the control group; the cells were observed using an optical microscope without administering the extract. The second set up used 25% concentration of the papaya seeds extract to be administered to the cell and was observed using an optical microscope. The next two set ups were also observed by an optical microscope with a concentration of 50% and 100% of the papaya seeds extract respectively, this concentrations were administered to the cells as well. All other conditions were controlled and observed. All set ups were repeated five times using 5 milliliters of the cell per trial.
 Statistical Results
The analysis of variance based on standard deviation clearly showed a coefficient of 1754.24257 for treatment which is greater than the tabulated variance based on PSD 1315.68192 at 5% level of confidence. This means that the treatments were significantly different at 5% level of significance. Furthermore, the observed PSD with coefficient of 36.27233 is lesser than the tabulated standard deviation of 41.88368 at 1% level of significance. Though, treatments were greatly significant at 5% level of confidence and not in 1% level of confidence. According to the analysis of variance statistical tool, 5% level of significance is still an excellent level of significance. Coefficient of variance of 56.15648 revealed that the data were clustered close to the mean. That is, the percentages of active cell in albino white mice sperm cell were of comparable number.

The Production of Aromatic Candles Using Waste Plastics as Substitute for Micro Wax

Antonio, Jude Trixie D.
Ebro, Edel Ain T.
Villanueva, Mary Grace A.
 Research Adviser:
Mrs. Ma. Regaele A. Olarte

            A necessity for cheaper and long-lasting candle motivated the researchers to think about using Polystyrene and LDPE (Low Density Polyethylene) plastics as substitutes for micro wax. The researchers used cheaper ingredients that could make a candle last longer than the normal commercially-released ones. The researchers constructed three different kinds of set-ups; set-up A with polystyrene plastics added up with orange extract for the scent, set-up B with LDPE plastics added up with the orange extract and lastly, set-up C, the controlled set-up with the micro wax added up with the orange extract too. There were significant differences between the experimental and controlled set-ups. Using plastics like these polystyrene and LDPE was better as to compare with the candles that were commercially released with micro wax. The second set-up (set-up B) was found to be the best alternative for candles for it was made of the LDPE plastics. Second was the first set-up (set-up A), because it was made of Polystyrene plastics. Last of all was the set-up C or the controlled set-up which was bought by the researchers and which was found out the worst among the three for it was the most expensive and had the shortest ability to last longer. The study proved that using different kinds of plastics, like what was used by the researchers was better than that of what was usual. The study showed not how useless but how useful and helpful plastics can be with the economy of the world.

The Effect of Clindamycin as an Effective Ingredient in Making Facial Soap

 Researcher: Aisa D. Lamdag
Research Adviser: Mrs. Ma. Regaele A. Olarte

Soap is something that should be in every personal emergency kit, but it’s one item that is often overlooked, one reason why the researcher chose this topic.  Nowadays, keeping things clean might be a lower priority for you during emergency but it’s something you really need to think about.  Soaps would not just clean your skin thus it would also make you feel comfortable.
The researcher made a simple and cheap homemade facial soap through the melt and pour soap process.  She used a microwave oven to melt the soap scraps.  The concentration of Clindamycin was then added to the liquid soap, stirred and poured to the soap molder, the same procedures were repeated for the different concentrations. The products were tested in terms of its pH level in washing the face and its hardness. 
As a result of completing the procedures, the researcher observed that the lowest concentration of Clindamycin has the hardest among the soaps produced.  It is also the lowest concentration that has a pH level which is good for the skin since a good facial soap ranges the pH level of 7.4-8.6 pH.
After the experiment and the laboratory testing, the researcher recommends to those who would do the same experiment to further investigate the physical and chemical properties of the soap.

The Effect of Varying Amount of Hydroponic Solutions to the Growth of Lettuce (Lactuca sativa) Plant in a Controlled Hydroponics System

Christian C. Chua
Research Adviser:
Mrs. Ma. Regaele A. Olarte

Conceptual Framework
The Experimentation and Set-ups of a Controlled Hydroponics System Using Different Amounts of Hydroponic Solution and Lettuce (Lactuca sativa) Plant

The hydroponics system designed by the researcher was adopted from the design of the Institute of Plant Breeding CSC-CA, University of the Philippines Los BaƱos. The cycle of the system is very easy to understand because the plants will be using the nutrients of the solution until they reach their full growth. The system is a labor free and self maintaining system because one can leave the system without monitoring it.
A summary of four set-ups is seen on the results. The tables, graphs and statistical analyses were presented to show if there was a significant difference in the growth of Lactuca Sativa (Lettuce) plant in the experimental and controlled hydroponics set-up. This was measured in terms of height of the plants in centimeters. The first set-up which was the controlled set-up showed how the plant normally grows when planted traditionally (planted on the soil) while the other three set-up showed the use of the controlled hydroponics solution with different amounts of solutions.
As what was expected by the researcher, the plants normally grew on the controlled set-up. Set-up A with 12.5 ml of solution didn’t satisfy the needed nutrients of the plants so as a result, the plants died on the 3rd week of the experiment. The other two set-ups, set-up B and set-up C with 25ml and 50ml of solutions respectively satisfy the needed nutrients of the plants so the plants grew normally just like on the controlled set-up.
The researcher used unpaired, two sample t-test to determine if there was a significant difference between the growth of lettuce (Lactuca sativa) planted on soil and on the controlled hydroponics system in terms of height in centimeters. Since the statistical units underlying the two samples being compared were non-overlapping. Based on the results of the statistical method used by the researcher, the researcher proved that there is no significant difference between using the controlled hydroponics system and the traditional planting method in yielding Lactuca Sativa (Lettuce) plant.
      The results of the experiment showed that 25ml and 50ml amount of solutions are applicable in growing Lactuca Sativa (Lettuce) plant.