Tuesday, March 8, 2016

Anthracnose Inhibiting Activity of Madre de Cacao (Gliricidia sepium) Leaf Extract on Post – harvest Tomato (Solanum lycopersicum)

Researchers: Tupas, Frederick King M., Forastero, Nicolette, Armas Alexia Marie L.,
 Lebrias, Maria Teddielyn Bernadette, and Posada, Elijah Rafael

Research Adviser: Mrs. Ma. Regaele A. Olarte

                  Anthracnose is a common and serious disease of plants in tropical region and humid areas. It is a fungus that causes heavy and deep damage to tomatoes making it easy to rot. Growth of Anthracnose (C. Coccodes) is most rapid at 80° F, although the fungus can cause infections over a wide range of temperatures (55°-95° F). Wet weather promotes disease development, and splashing water in the form of rain or overhead irrigation favors the spread of the disease. Colonies as observed by the researchers are usually dark pigmented with white aerial vegetative part of a fungus which is called mycelium. Consisting of numerous black compact mass of hardened fungal mycelium containing food reserves (sclerotia) and light brown colored conidial masses, reverse is a dark brown conidial masses. It is also a result in wide range of symptoms like leaf spots, defoliation and distortion in many different kinds of plants. One of the plants that is affected by this kind of fungi are tomatoes.
  The researchers have come up with the idea of using Madre de Cacao (Gliricidia sepium) leaf extract on post-harvest Tomatoes (Solanum lycopersicum). The study ascertained that the medicarpin content of the said plant is an effective treatment for fungi. Since Gliricidia has already proven its power on fungal infections, the researchers conducted several experimentation and trials to figure out its effectiveness; with this, Anthracnose can now be prevented and cured without the help of commercially known anti-fungal treatment.

Mucus from Brown Garden Snail (Helix aspersa) as Desiccating Agent to Absorb Moisture


Ivy Lane Erguiza, Priscila Aguila, Janine Sabado, Honey Balbastro

Research Adviser:


3rd Place DepEd Regional Science Fair 2014
3rd Place National Science and Youth Congress 2014
4th Runner Up National Science Quest 2015

Among the reasons for food shortage in the country are pest infestation in crops and
damages in the shipping of goods. Upon seeking ways to exterminate these unwanted
factors, the researcher came up with the idea of targeting these two problems. The
researcher investigated the hygroscopic property of Brown Garden Snail (Helix aspersa)
slime and converted it into a very useful product.
The organic desiccant was derived from the extraction of slime obtained from the body
secretions of the snail. The following experimental set-ups had been made: 5.6 grams wet
slime and 1.6 grams wet filter paper; 5.6 grams dry slime and 1.6 grams wet filter paper.
For the control set-up, 5.6 grams commercial desiccant and 1.6 grams wet filter paper
were used as positive control; 1.6 grams wet filter paper without treatment was used as
the negative control.
One-way Analysis of Variance (ANOVA) for independent groups showed that there was an
overall significant difference among the group means, at P <.0001. The results of post-hoc
Tukey's HSD tests showed that all points of comparison were significant, P<.01. The study
proved that the organic desiccant obtained from Snail (Helix aspersa) slime could be
effective moisture absorbent, especially the wet mucus.


Snail; desiccant; Helix aspersa; hygroscopic property


Wound Healing Effect of Ashitaba (Angelica keiskei) Extract on Albino Mice

Bryant ALTERADO, Reggie BAGATELA, Elijah Jeremie SORIANO

S.Y. 2014-2015


            Due to the high cost of the commercial medicines, people are incited to discover and seek for an alternative way to cure particular ailments by the utilization of medicinal plants. People in rural places and those who cannot afford to buy expensive topical treatment for skin diseases need to discover what nature can offer as alternative medication. Ashitaba (Angelica keiskei) is a plant that is abundant in many regions of Asia. It was first used in Japan as herbal tea for treatment of different diseases, and it multiplied rapidly, particularly in the Philippines. Subsequently, the researchers studied the beneficial uses of the Ashitaba (Angelica keiskei) leaves, specifically its capability to treat wounds.
            A total of four sets of experiments are prepared. Three (3) mice per four (4) different kinds of set-ups. Pure Ashitaba (Angelica keiskei) extract and decocted Ashitaba (Angelicakeiskei) extract served as the experimental set-ups. Betadine (Povidone-iodine) and the untreated set-up served as the control set-ups.
           The results of the experiment revealed that among all the treatments, 100% Angelica keiskei pure extract had the highest rate of wound healing in albino mice. Next were the Povidone-iodine and the decocted extract of Angelica keiskei, which were at the same level of efficiency. Statistical analysis showed that there’s no significant difference between the experimental set-ups (Ashitaba leaf and stem pure extract, and decocted extract) and the control set-up (Povidone-iodine) in terms of wound healing rate.

Utilization of Aratilis (Muntingiacalabura) as Feedstock for Bioethanol

Macasinag, John Paulo H.; Joson, Jonas S.; Pante, Jonel B.
Magpale, Vincent; Ybalez, Kent Nehemiah

 Research Adviser: Mrs. Ma. Regaele A. Olarte

CHAMPION - National Science and Youth Congress
Youth Greenovation 2014 - Applied Science Category
University of the Philippines, Los Banos

   Production of green energy from indigenous materials has played an essential role in recent days due to the depletion of non-renewable energy resources. Viable alternative sources of energy have long been searched for in the midst of the increasing demand of for fossil fuels. People are already upset by the high fuel prices, and in reality they are likely to go much higher in the future. Thus, an alternative fuel source such as bioethanol can save the consumption and dependency on fuel wood.
Aritilis (Muntingiacalabura)is a fruit-bearing tree that is abundant in many regions of the Philippines, and because of its plentiful growth, it is sometimes considered as nuisance whenever it produces too much waste on the ground where it falls off. This made the researchers decide to use aratilis fruit as the main material in producing bioethanol considering that it is rich in starch that is convertible to sugar, which is then processed to become bioethanol.
Background of the Study
Bioethanol is a renewable fuel source. It is produced from plants that process and store energy from the sun. In a sustainable cropping system, plant feedstock could be produced year after year. Regions of the world that were without crude oil deposits could consider fuel farming as a long term solution to offset their energy needs and foreign oil dependency.
 Bioethanol can be made from common crops such as sugar cane, sweet sorghum, and the like; unlike petroleum which is produced from agricultural feedstock. It burns more clearly and produces very little amount of carbon monoxide which can help in reducing pollution in our country. It is also renewable, making it more practical and affordable.
Aratilis fruit is a starchy substrate which can be utilized for the production of green energy, bio-ethanol.Ethanol from starch is generally produced by the fermentation process.In order to produce ethanol from starchy material, the starch must first be converted into sugars. 

The Exterminating Effect of Cane Toad (Bufo marinus) Bufotoxin Crude Extract against Rice Black Bug (Scotinophara coarctata)

Bermejo, Aldin M.
Montante, Samboy Jim B.
Peneyra, Rona Moneth M.
Venus Angeline Baldonaza
Shanelli Deviterbo

Research Adviser:

Ma. Regaele A. Olarte

1st Place Winning Entry in the Divison, Regional, and National Levels
Candidate to the INTERNATIONAL Delegation Team for ISEF 2012-2013


             One of the reasons for rice shortage in the country is pest infestation, specifically, by Rice Black Bug. The researchers were challenged to come up with another method of combating Rice Black Bugs in the rice field, without altering the balance of nature. Upon seeking ways to exterminate these unwanted pests, the researchers investigated the exterminating property of Cane Toad (Bufo marinus) crude extract against Rice Black Bug. The pesticide was derived from the extraction of the 22.5 grams of crude extract. The following experimental set-ups had been made: 30% crude extract and 70% corn oil solution (0.3 grams of crude extract and 0.7grams corn oil solution); 50% crude extract and 50% corn oil solution (0.5 grams of crude extract and 0.5 grams corn oil solution); 70% crude extract and 30% corn oil solution (0.7 grams of crude extract and 0.3 grams corn oil solution.); and 100% crude extract (1 gram of crude extract). Using the different concentrations, the effectiveness was tested to the Rice Black Bug (Scotinophara coarctata), in terms of mortality rate. T- test showed that there was no significant difference between the experimental product and the commercial bug killer in terms of the pest’s mortality rate, at 0.05 level of significance. The results of experimentation and data analysis showed that the experimental product was just as effective as the commercial bug killer in eradicating pests. Thus, the organic pesticide obtained from Cane Toad (Bufo marinus) crude extract could be an alternative to the commercial bug killer.

Friday, October 7, 2011

Multi-Colored Fabric Dye From Talisay (Terminalia catappa) Leaves Extract

Pauline Mariz Manuel
Marjorie Marmeto
Jiezel Ann Sescar
Mrs. Ma. Regaele Olarte (Research Adviser)
Statement of the Problem      
The researchers aimed to determine if the Talisay (Terminalia catappa) leaves extract could be developed as a multi-colored fabric dye.
      Specifically, the study sought to answer the following questions:
1.      Can Talisay (Terminalia catappa) leaves extract be developed as a multi-colored dye?
2.      Is there a significant difference between the experimental and control set-ups in terms of:
a.  Reaction to acid
b.  Reaction to base
c.  pH level
d.  Color fastness

Table 1
Frequency Distribution of Yielded Results
in terms of Color


 Table 2

Comparison of Mean and T-test Result for 
Talisay and Commercial Fabric Dye




Thursday, October 6, 2011

Akapulko (Cassia alata L.) Seeds as Purgative Extender for Pulvoron

Akapulvoron: A Sensory Analysis
Akapulko (Cassia alata L.) Seeds as Purgative Extender for Pulvoron
Karl Ericson Landrito
Rochelle Gel Asilo
Ludy Anne Castrence
Maegan Morales
Keith Louise R. Salazar
Research Adviser:
Mrs. Ma. Regaele A. Olarte
A.   Background of the Study
          Children during their early stage of learning are curious on how things around them work. They always want to play outdoor games such as tumbang preso, patintero, piko, jolens, pogs, and many other games with their playmates. These games require touching and a lot of barefoot running. Children do not tend to know that running barefooted will cause the formation of ascaris and other parasitic worms that might infect their intestines. Malnutrition surrounds our country today; a lot of people, most especially the children, were the victims of it. One of the most common causes of malnutrition of children is worm infestation.
          Worm infestation can result if there happened to be a direct or indirect contact with the feces of an infected person. It can be dispersed when the feces of an infected organism are used as fertilizers in farmlands that are now converted into urban type of community. The kids that are living in that converted area can accumulate the infection that the former farmland has. 
Children that are infected can have parasitic worms inside their bodies. They can eventually experience malnutrition and other health deficiencies if not treated. Purgative medicines are cheap in cost but children dislike the taste because of its bitterness. The researchers came up with this project due to the desire of helping the parents of infected children in convincing their kids to take good-tasting purgative remedy that is cheaper in cost and can be done at home. The researchers found out that pounded and boiled akapulko seeds are used as an effective purgative agent.  Akapulko is an herbal plant that can be used in curing skin, stomach, lung, and mouth problems, and is well known for its sudorific, diuretic and purgative characteristics. It can be ingested without having any side effect or contraindications. The researchers thought of pulvoron, being a healthy, delicious, and attractive treat for all. The akapulko seeds were consider as extender in place of the traditional rice crisps, nuts, or nougats in the pulvoron. Established that akapulvoron could be an effective and safe purgative treat, the researchers intended to conduct a sensory analysis of the product to determine if it would be generally accepted by the public.

Wednesday, October 5, 2011

Makahiya (Mimosa pudica) Root Extract as an Alternative Ovicide against Dengue Mosquito (Aedes aegypti) Eggs

Dumayas, Diana Rose P.
Geroche, Sheila Mae T.
Pinote, Lorraine Angela D.
Mrs. Ma. Regaele A. Olarte
(Research Adviser)

Significance of the Study
The augmenting number of Dengue cases not only in our country but also to other countries all over the world experiencing the abnormal change in weather is not of recent origin to us. Along with this difficult situation, there are two-winged, dipterous insects that hatch their eggs for a short time, reducing the size of their larvae and become “nervous-biters”.    In that case, they are harder to intercept in flight that is why it is more advisable to focus on the places where they lay their eggs.
      The researchers conducted this study in order to lessen the proliferation of mosquitoes or prevent the eggs from hatching using the Makahiya Mimosa pudica roots extract as an ovicide. As the experiment was proven to be effective, it is relevant to propagate the plant for mass production and communicate the results to other people. It is also significant to conduct experiments about the other potentialities of  the said plant, such as the anti fertility property of its root extract which was  proven in Albino white mice sperm cells according to Dr. Godofredo Umali-Stuart of University of Santo Tomas.