Tuesday, March 8, 2016

The Exterminating Effect of Cane Toad (Bufo marinus) Bufotoxin Crude Extract against Rice Black Bug (Scotinophara coarctata)

Researchers:
Bermejo, Aldin M.
Montante, Samboy Jim B.
Peneyra, Rona Moneth M.
Venus Angeline Baldonaza
Shanelli Deviterbo

Research Adviser:

Ma. Regaele A. Olarte

1st Place Winning Entry in the Divison, Regional, and National Levels
Candidate to the INTERNATIONAL Delegation Team for ISEF 2012-2013

Abstract

             One of the reasons for rice shortage in the country is pest infestation, specifically, by Rice Black Bug. The researchers were challenged to come up with another method of combating Rice Black Bugs in the rice field, without altering the balance of nature. Upon seeking ways to exterminate these unwanted pests, the researchers investigated the exterminating property of Cane Toad (Bufo marinus) crude extract against Rice Black Bug. The pesticide was derived from the extraction of the 22.5 grams of crude extract. The following experimental set-ups had been made: 30% crude extract and 70% corn oil solution (0.3 grams of crude extract and 0.7grams corn oil solution); 50% crude extract and 50% corn oil solution (0.5 grams of crude extract and 0.5 grams corn oil solution); 70% crude extract and 30% corn oil solution (0.7 grams of crude extract and 0.3 grams corn oil solution.); and 100% crude extract (1 gram of crude extract). Using the different concentrations, the effectiveness was tested to the Rice Black Bug (Scotinophara coarctata), in terms of mortality rate. T- test showed that there was no significant difference between the experimental product and the commercial bug killer in terms of the pest’s mortality rate, at 0.05 level of significance. The results of experimentation and data analysis showed that the experimental product was just as effective as the commercial bug killer in eradicating pests. Thus, the organic pesticide obtained from Cane Toad (Bufo marinus) crude extract could be an alternative to the commercial bug killer.

10 comments:

  1. where can we find or purchase cane toads?

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  2. You can purchase it from UPLB Institute of Biology.

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  3. Where can we find the Rice Black Bugs? And also if we were to purchase Cane Toads at UPLB where is the exact place that we have to go to?

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  4. Can we ask for your number? We just want to inquire about your research and we really need your help. Hope this could reach you.

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  5. How did you extract the crude from the cane toads? Will this extraction kill the frog? How did you conduct the study? I just want to base my Research methods to yours so I really need your help. Thank you!

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  6. How did you do in your experimentation. I mean how did you make a pesticide out of it?

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  7. Full doc. please
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  9. what are the different laboratory techniques/assays used in the research?

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